Observer 7 Microscopy Papers – Page 6

⭐ Foundational

Interception of host angiogenic signalling limits mycobacterial growth.

Nature 2015 📊 253

Pathogenic mycobacteria induce the formation of complex cellular aggregates called granulomas that are the hallmark of tuberculosis. Here we examine the development and consequences of...

⭐ Foundational

PD-1 promotes immune exhaustion by inducing antiviral T cell motility paralysis.

The Journal of experimental medicine 2013 📊 265

Immune responses to persistent viral infections and cancer often fail because of intense regulation of antigen-specific T cells-a process referred to as immune exhaustion. The...

Fluorescence Microscopy Total Internal Reflection Fluorescence Microscopy Two-Photon Excitation Microscopy Z-Stack Imaging Axio Observer Leica SP5 Leica SP5 Confocal Observer 7 SP5 Ti Zeiss Axio Observer NIS-Elements Alexa Fluor 405 Alexa Fluor 568 Alexa Fluor 647 Alexa Fluor 700 APC CFP Cy7 DAPI DyLight FITC GFP Pacific Blue PerCP Rhodamine Blocking Fixation Immunohistochemistry Methanol Fixation Permeabilization Vibratome Baby Hamster Kidney BHK-21 Chinese Hamster Ovary Primary Dendritic Cells Primary Macrophages Primary T Cells Vero (African Green Monkey Kidney)Leica SP5 Confocal ImageJ Imaris NIS-Elements National Institute of Neurological Disorders and Stroke National Institutes of Health BioLegend Cell Signaling Technology eBioscience ibidi Invitrogen Jackson ImmunoResearch Sigma-Aldrich Vector Laboratories Guinea Mouse Rabbit Rat Spectra-Physics Mai Tai HP Ti:Sapphire Andor EMCCD Leica 20x/1.0 NA Nikon Apo TIRF 100x oil

⭐ Foundational

Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibited and Activated.

Cell 2017 📊 283

Cytoplasmic dynein-1 binds dynactin and cargo adaptor proteins to form a transport machine capable of long-distance processive movement along microtubules. However, it is unclear why...

Confocal Laser Scanning Microscopy Cryo-Electron Microscopy Fluorescence Microscopy Immunofluorescence Microscopy Live Cell Imaging Total Internal Reflection Fluorescence Microscopy Axio Observer Observer 7 Ti Titan Zeiss Lightsheet Z.1 Light Sheet Escherichia coli MicroManager Alexa Fluor 488 Alexa Fluor 647 DAPI DyLight EdU GFP Blocking Cell Culture Electroporation Fixation Glutaraldehyde Immunofluorescence Live Imaging Methanol Fixation Transduction Transfection Embryonic Stem Cell HeLa (Henrietta Lacks)Zeiss Lightsheet Z.1 Light Sheet Digital Micrograph EMAN2 Fiji PyMOL RELION UCSF Chimera MicroManager GraphPad Prism R INSERM Max Planck Max Planck Institute Max Planck Institute of Molecular Physiology MRC Laboratory of Molecular Biology Université Paris Descartes Abcam Bio-Rad Gibco Invitrogen Life Technologies Lonza Merck Millipore New England Biolabs Promega Qiagen Roche Santa Cruz Biotechnology Sigma-Aldrich Thermo Fisher Scientific VWR Donkey Goat Mouse Rabbit Rat Sheep Coherent Sapphire diode Andor EMCCD CCD EMCCD Gatan CCD Hamamatsu Orca Flash 4.0 PCO sCMOS Thermo Fisher Falcon III 60x/1.42 NA Zeiss 63x/1.4 NA

💾 Data 🏷️ 11 RRIDs

⭐ Foundational

Visualizing B cell capture of cognate antigen from follicular dendritic cells.

The Journal of experimental medicine 2009 📊 290

The prominent display of opsonized antigen by follicular dendritic cells (FDCs) has long favored the view that they serve as antigen-presenting cells for B cells....

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⭐ Foundational

Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues.

Nature biotechnology 2019 📊 413

Spatial mapping of proteins in tissues is hindered by limitations in multiplexing, sensitivity and throughput. Here we report immunostaining with signal amplification by exchange reaction...

Confocal Laser Scanning Microscopy Expansion Microscopy Z-Stack Imaging Axio Observer Leica SP5 Leica SP5 Confocal LSM 710 Observer 7 SP5 Zeiss Axio Observer Zeiss LSM 710 Confocal CellProfiler Fiji Imaris LAS X MATLAB OMERO Python U-Net ATTO 488 ATTO 565 Cy5 DAPI EdU Antigen Retrieval Blocking Cell Culture Expansion Microscopy Fixation Immunostaining Microtome Paraformaldehyde Fixation Permeabilization Whole Mount HeLa (Henrietta Lacks)Primary Astrocytes Primary Neurons Leica SP5 Confocal Zeiss LSM 710 Confocal CellProfiler Imaris OMERO U-Net LAS X MATLAB Python Beth Israel Deaconess Medical Center Harvard Medical School Harvard University Massachusetts Institute of Technology MIT Modelica Association University of Washington Wyss Institute for Biologically Inspired Engineering Agilent Bio-Rad Cytiva Gibco ibidi Invitrogen Jackson ImmunoResearch Life Technologies Merck Millipore New England Biolabs Qiagen Sigma-Aldrich Vector Laboratories Goat Mouse Rabbit Hamamatsu ORCA R2 Leica PMT PerkinElmer PMT 10x/0.45 NA air 20x/0.8 NA air 20x/0.80 NA air Olympus 20x/0.75 NA air

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⭐ Foundational

Imaging cellular ultrastructures using expansion microscopy (U-ExM).

Nature methods 2019 📊 458

Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion...

3D Imaging Confocal Laser Scanning Microscopy Cryo-Electron Microscopy Deconvolution Microscopy Direct Stochastic Optical Reconstruction Microscopy Expansion Microscopy Immunofluorescence Microscopy Stimulated Emission Depletion Microscopy Stochastic Optical Reconstruction Microscopy Super-Resolution Microscopy Z-Stack Imaging Axio Observer Leica SP8 Confocal Observer 7 SP8 Zeiss Axio Observer Zeiss Lightsheet Z.1 Light Sheet Chlamydomonas reinhardtii NIS-Elements Alexa Fluor 488 Alexa Fluor 568 Cy3 Hoechst 33342 MitoTracker Blocking Expansion Microscopy Fixation Glutaraldehyde Immunofluorescence Immunostaining Paraformaldehyde Fixation Permeabilization African Green Monkey Kidney COS-7 Human Osteosarcoma U2OS Leica SP8 Confocal Zeiss Lightsheet Z.1 Light Sheet Digital Micrograph Fiji Huygens ImageJ ThunderSTORM NIS-Elements MATLAB CNRS EPFL Institute of Molecular Genetics Massachusetts Institute of Technology MIT Université de Strasbourg University of Geneva University of Würzburg Abcam Invitrogen Jackson ImmunoResearch Roche Sigma-Aldrich Thermo Fisher Scientific Goat Mouse Rabbit Rat Cobolt Skyra DPSS Andor Ixon Ultra Andor sCMOS Andor Zyla 4.CCD Gatan CCD Leica 63x/1.4 NA oil Olympus UPLSAPO 60x Zeiss 100x/1.4 NA oil Zeiss alpha Plan-Apochromat 100x Zeiss LD C-Apochromat 63x

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⭐ Foundational

Democratising deep learning for microscopy with ZeroCostDL4Mic.

Nature communications 2021 📊 456

Abstract Deep Learning (DL) methods are powerful analytical tools for microscopy and can outperform conventional image processing pipelines. Despite the enthusiasm and innovations fuelled by...

Confocal Laser Scanning Microscopy DNA Points Accumulation for Imaging in Nanoscale Topography Live Cell Imaging Single-Molecule Localization Microscopy Spinning Disk Confocal Microscopy Stochastic Optical Reconstruction Microscopy Structured Illumination Microscopy Super-Resolution Microscopy Widefield Fluorescence Microscopy Z-Stack Imaging Axio Observer CSU-W1 Leica SP8 Leica SP8 Confocal N-STORM Nikon N-STORM Nikon N-STORM Super-Resolution Observer 7 SP8 Ti Yokogawa CSU-W1 Yokogawa CSU-W1 Spinning Disk Zeiss Axio Observer NIS-Elements SlideBook Alexa Fluor 488 Alexa Fluor 594 Alexa Fluor 647 ATTO 655 GFP Hoechst 33342 mRFP Phalloidin RFP Cell Culture Clustered Regularly Interspaced Short Palindromic Repeats Fixation Glutaraldehyde Lentiviral Live Imaging Permeabilization HeLa (Henrietta Lacks)Human Breast Adenocarcinoma MDA-MB-231 Human Osteosarcoma U2OS Leica SP8 Confocal Nikon N-STORM Super-Resolution Yokogawa CSU-W1 Spinning Disk Fiji ImageJ Imaris StarDist ThunderSTORM TrackMate U-Net YOLOv NIS-Elements SlideBook Python Åbo Akademi University Akademi University CNRS Francis Crick Institute Imperial College London Israel Institute of Technology Max Planck Max Planck Institute Max Planck Institute for Molecular Cell Biology and Genetics Max Planck Institute for Physics of Complex Systems Newcastle University The Classical Association University College London University Frankfurt University of Turku University of Turku and Åbo Akademi University Cytiva Gibco Life Technologies MatTek Santa Cruz Biotechnology Sigma-Aldrich Thermo Fisher Scientific Mouse Rabbit EMCCD PCO sCMOS Photometrics EMCCD Photometrics evolve PMT

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⭐ Foundational

Locally renewing resident synovial macrophages provide a protective barrier for the joint.

Nature 2019 📊 528

Macrophages are considered to contribute to chronic inflammatory diseases such as rheumatoid arthritis1. However, both the exact origin and the role of macrophages in inflammatory...

Brightfield Microscopy Confocal Laser Scanning Microscopy Fluorescence Microscopy Immunofluorescence Microscopy Light Sheet Fluorescence Microscopy Spinning Disk Confocal Microscopy Transmission Electron Microscopy Axio Observer CSU-X1 LaVision BioTec UltraMicroscope Light Sheet Observer 7 Ti UltraMicroscope ZEN ZEN Blue Alexa Fluor 488 Alexa Fluor 594 Alexa Fluor 647 Alexa Fluor 700 APC BV421 Cy5 Cy5.5 DAPI DsRed EdU EGFP FITC GFP mCherry PerCP Phalloidin SYTOX SYTOX Blue tdTomato Blocking Cryosectioning Fixation Glutaraldehyde Immunofluorescence Microtome Optimal Cutting Temperature Embedding Paraformaldehyde Fixation Permeabilization Tissue Clearing Primary Endothelial Cells Primary Macrophages Primary Monocytes Primary Neutrophils LaVision BioTec UltraMicroscope Light Sheet Huygens Imaris ZEN ZEN Blue GraphPad Prism Alexander University Institut de Génétique Humaine Leibniz Research Institute of Molecular Pharmacology Research Institute of Radiology Spanish National Centre for Cardiovascular Research Universitätsklinikum Erlangen BioLegend Dako Invitrogen Qiagen Sigma-Aldrich Donkey Rabbit Rat Andor sCMOS Leica HyD Photometrics Evolve 512 Yokogawa EMCCD Leica HCX PL APO 100x oil Zeiss LD C-Apochromat 63x water 445/50 bandpass 525/50 bandpass 605/70 bandpass

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⭐ Foundational

Probing protein heterogeneity in the plasma membrane using PALM and pair correlation analysis.

Nature methods 2011 📊 580

Photoactivated localization microscopy (PALM) is a powerful approach for investigating protein organization, yet tools for quantitative, spatial analysis of PALM datasets are largely missing. Combining...

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⭐ Foundational

The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.

Nature communications 2019 📊 503

Abstract Specialized epitope tags are widely used for detecting, manipulating or purifying proteins, but often their versatility is limited. Here, we introduce the ALFA-tag, a...

3D Imaging Confocal Laser Scanning Microscopy DNA Points Accumulation for Imaging in Nanoscale Topography Epifluorescence Microscopy Immunofluorescence Microscopy Stimulated Emission Depletion Microscopy Super-Resolution Microscopy Axio Observer IX83 Observer 7 Olympus IX83 Ti Zeiss Lightsheet Z.1 Light Sheet Escherichia coli Imspector APC ATTO 488 BV421 DAPI EGFP FITC GFP mScarlet Blocking Fixation Glutaraldehyde Immunofluorescence Lipofection Methanol Fixation Paraformaldehyde Fixation Permeabilization Transfection African Green Monkey Kidney COS-7 HeLa (Henrietta Lacks)NIH 3T3 Mouse Fibroblast Primary B Cells Olympus IX83 Zeiss Lightsheet Z.1 Light Sheet ImageJ Imspector GraphPad Prism LMU Munich Lund University Max Planck Max Planck Institute Max Planck Institute of Biochemistry Stockholm University Universitätsmedizin Göttingen University Hospital and Clinics BD Biosciences BioLegend ibidi Merck Sigma-Aldrich Thermo Fisher Scientific Goat Mouse Rabbit Coherent Sapphire diode Toptica iBeam smart APD Chroma ZET488/10x Chroma ZET561/10x

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Observer 7

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