Axio Observer Microscopy Papers – Page 7

⭐ Foundational

Imaging cellular ultrastructures using expansion microscopy (U-ExM).

Nature methods 2019 📊 458

Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion...

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⭐ Foundational

Democratising deep learning for microscopy with ZeroCostDL4Mic.

Nature communications 2021 📊 456

Abstract Deep Learning (DL) methods are powerful analytical tools for microscopy and can outperform conventional image processing pipelines. Despite the enthusiasm and innovations fuelled by...

Confocal Laser Scanning Microscopy DNA Points Accumulation for Imaging in Nanoscale Topography Live Cell Imaging Single-Molecule Localization Microscopy Spinning Disk Confocal Microscopy Stochastic Optical Reconstruction Microscopy Structured Illumination Microscopy Super-Resolution Microscopy Widefield Fluorescence Microscopy Z-Stack Imaging Axio Observer CSU-W1 Leica SP8 Leica SP8 Confocal N-STORM Nikon N-STORM Nikon N-STORM Super-Resolution Observer 7 SP8 Ti Yokogawa CSU-W1 Yokogawa CSU-W1 Spinning Disk Zeiss Axio Observer NIS-Elements SlideBook Alexa Fluor 488 Alexa Fluor 594 Alexa Fluor 647 ATTO 655 GFP Hoechst 33342 mRFP Phalloidin RFP Cell Culture Clustered Regularly Interspaced Short Palindromic Repeats Fixation Glutaraldehyde Lentiviral Live Imaging Permeabilization HeLa (Henrietta Lacks)Human Breast Adenocarcinoma MDA-MB-231 Human Osteosarcoma U2OS Leica SP8 Confocal Nikon N-STORM Super-Resolution Yokogawa CSU-W1 Spinning Disk Fiji ImageJ Imaris StarDist ThunderSTORM TrackMate U-Net YOLOv NIS-Elements SlideBook Python Åbo Akademi University Akademi University CNRS Francis Crick Institute Imperial College London Israel Institute of Technology Max Planck Max Planck Institute Max Planck Institute for Molecular Cell Biology and Genetics Max Planck Institute for Physics of Complex Systems Newcastle University The Classical Association University College London University Frankfurt University of Turku University of Turku and Åbo Akademi University Cytiva Gibco Life Technologies MatTek Santa Cruz Biotechnology Sigma-Aldrich Thermo Fisher Scientific Mouse Rabbit EMCCD PCO sCMOS Photometrics EMCCD Photometrics evolve PMT

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⭐ Foundational

Real-Time Imaging Reveals Local, Transient Vascular Permeability, and Tumor Cell Intravasation Stimulated by TIE2hi Macrophage-Derived VEGFA.

Cancer discovery 2015 📊 522

Abstract Dissemination of tumor cells is an essential step in metastasis. Direct contact between a macrophage, mammalian-enabled (MENA)–overexpressing tumor cell, and endothelial cell [Tumor MicroEnvironment...

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⭐ Foundational

Locally renewing resident synovial macrophages provide a protective barrier for the joint.

Nature 2019 📊 528

Macrophages are considered to contribute to chronic inflammatory diseases such as rheumatoid arthritis1. However, both the exact origin and the role of macrophages in inflammatory...

Brightfield Microscopy Confocal Laser Scanning Microscopy Fluorescence Microscopy Immunofluorescence Microscopy Light Sheet Fluorescence Microscopy Spinning Disk Confocal Microscopy Transmission Electron Microscopy Axio Observer CSU-X1 LaVision BioTec UltraMicroscope Light Sheet Observer 7 Ti UltraMicroscope ZEN ZEN Blue Alexa Fluor 488 Alexa Fluor 594 Alexa Fluor 647 Alexa Fluor 700 APC BV421 Cy5 Cy5.5 DAPI DsRed EdU EGFP FITC GFP mCherry PerCP Phalloidin SYTOX SYTOX Blue tdTomato Blocking Cryosectioning Fixation Glutaraldehyde Immunofluorescence Microtome Optimal Cutting Temperature Embedding Paraformaldehyde Fixation Permeabilization Tissue Clearing Primary Endothelial Cells Primary Macrophages Primary Monocytes Primary Neutrophils LaVision BioTec UltraMicroscope Light Sheet Huygens Imaris ZEN ZEN Blue GraphPad Prism Alexander University Institut de Génétique Humaine Leibniz Research Institute of Molecular Pharmacology Research Institute of Radiology Spanish National Centre for Cardiovascular Research Universitätsklinikum Erlangen BioLegend Dako Invitrogen Qiagen Sigma-Aldrich Donkey Rabbit Rat Andor sCMOS Leica HyD Photometrics Evolve 512 Yokogawa EMCCD Leica HCX PL APO 100x oil Zeiss LD C-Apochromat 63x water 445/50 bandpass 525/50 bandpass 605/70 bandpass

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⭐ Foundational

Fast and sensitive GCaMP calcium indicators for imaging neural populations.

Nature 2023 📊 656

Abstract Calcium imaging with protein-based indicators 1,2 is widely used to follow neural activity in intact nervous systems, but current protein sensors report neural activity...

Calcium Imaging Confocal Laser Scanning Microscopy Fluorescence Correlation Spectroscopy Fluorescence Recovery After Photobleaching Immunofluorescence Microscopy Spinning Disk Confocal Microscopy Two-Photon Excitation Microscopy Axio Imager Axio Observer CSU-X1 Nikon Ti Ti Yokogawa CSU-X1 Spinning Disk Zeiss Axio Observer Drosophila melanogaster Escherichia coli MetaMorph ScanImage Alexa Fluor 488 Alexa Fluor 594 Cy3 DAPI EdU EGFP GCaMP GCaMP6 GCaMP6s GFP jGCaMP7 jGCaMP8 mCherry mEmerald RFP tdTomato Adeno-Associated Virus Cell Culture Electroporation Fixation Immunofluorescence Immunostaining Paraformaldehyde Fixation Transduction Transfection Embryonic Stem Cell Primary Neurons Yokogawa CSU-X1 Spinning Disk ilastik PyMOL MetaMorph ScanImage Excel MATLAB Python Allen Institute Allen Institute for Neural Dynamics HHMI Howard Hughes Medical Institute Janelia Research Campus Ministry of Defence Princeton University University of California University of Maryland School of Medicine Addgene Agilent Bio-Rad Invitrogen Lonza MatTek Millipore New England Biolabs Sigma-Aldrich Takara Bio Thermo Fisher Scientific Tocris Chicken Goat Mouse Rabbit Rat Coherent Chameleon Ultra Spectra-Physics Mai Tai Ti:Sapphire Andor EMCCD Andor iXon 897 Andor Ixon Ultra EMCCD GaAsP Nikon EMCCD

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⭐ Foundational

The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.

Nature communications 2019 📊 503

Abstract Specialized epitope tags are widely used for detecting, manipulating or purifying proteins, but often their versatility is limited. Here, we introduce the ALFA-tag, a...

3D Imaging Confocal Laser Scanning Microscopy DNA Points Accumulation for Imaging in Nanoscale Topography Epifluorescence Microscopy Immunofluorescence Microscopy Stimulated Emission Depletion Microscopy Super-Resolution Microscopy Axio Observer IX83 Observer 7 Olympus IX83 Ti Zeiss Lightsheet Z.1 Light Sheet Escherichia coli Imspector APC ATTO 488 BV421 DAPI EGFP FITC GFP mScarlet Blocking Fixation Glutaraldehyde Immunofluorescence Lipofection Methanol Fixation Paraformaldehyde Fixation Permeabilization Transfection African Green Monkey Kidney COS-7 HeLa (Henrietta Lacks)NIH 3T3 Mouse Fibroblast Primary B Cells Olympus IX83 Zeiss Lightsheet Z.1 Light Sheet ImageJ Imspector GraphPad Prism LMU Munich Lund University Max Planck Max Planck Institute Max Planck Institute of Biochemistry Stockholm University Universitätsmedizin Göttingen University Hospital and Clinics BD Biosciences BioLegend ibidi Merck Sigma-Aldrich Thermo Fisher Scientific Goat Mouse Rabbit Coherent Sapphire diode Toptica iBeam smart APD Chroma ZET488/10x Chroma ZET561/10x

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⭐ Foundational

Deep learning enables cross-modality super-resolution in fluorescence microscopy.

Nature methods 2019 📊 714

We present deep-learning-enabled super-resolution across different fluorescence microscopy modalities. This data-driven approach does not require numerical modeling of the imaging process or the estimation of...

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⭐ Foundational

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.

Frontiers in molecular neuroscience 2013 📊 731

Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Here we describe red, single-wavelength GECIs, "RCaMPs," engineered from circular permutation of the thermostable...

Calcium Imaging Confocal Laser Scanning Microscopy Differential Interference Contrast Microscopy Fluorescence Correlation Spectroscopy Fluorescence Microscopy Optogenetics Axio Observer N-SIM Caenorhabditis elegans Drosophila melanogaster Escherichia coli Fiji GraphPad Prism ImageJ MATLAB Cy3 EdU EGFP GCaMP GFP mCherry mRuby Oregon Green BAPTA R-GECO RCaMP RFP Adeno-Associated Virus Co-culture Lentiviral Transduction Transfection Human Embryonic Kidney 293 Primary Astrocytes Axio Observer N-SIM ImageJ GraphPad Prism LabVIEW MATLAB Howard Hughes Medical Institute Janelia Farm Corning Invitrogen Life Technologies Lonza Millipore Qiagen Roche Takara Bio Coherent Chameleon Ti:Sapphire Andor EMCCD APD CCD Photometrics CCD Photometrics CoolSnap PicoQuant APD Princeton Instruments CCD Roper APD Roper CCD Semrock APD 40x/0.8 NA 60x/1.2 NA Zeiss 20x/0.85 NA Zeiss Plan-Neofluar 25x 647/57 bandpass Semrock FF01-468

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